In vitro growth and content of vincristine and vinblastine of Catharanthus roseus L. hairy roots in response to precursors and elicitors

Catharanthus roseus L. is a medicinal plant that produces numerous indole terpenoid alkaloids, including vincristine and vinblastine, which are used for cancer treatment. The effect of specified precursors (L-phenylalanine, L-tyrosine) elicitors (chitosan, methyl jasmonate) on C. hairy roots (CHR) growth has been examined in order to increase the content vinblastine. Our results showed CHR generated by an Agrobacterium rhizogenes strain isolated Vietnam was capable producing both vinblastine when subjected precursors, but only exposed elicitors. However, were evaluated have increasing accumulation TIAs CHR. In particular, use required more time find appropriate induction conditions, while gave outstanding efficiency treatment with 1 µM phenylalanine. greatest yields (51.99 µg g-1 DW) (699.92 obtained 7th week (with 0.306 g DW biomass). This result first we might boost levels our clone A. rhizogenes.